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Enzymatic Cell Dissociation Agents
Trypsin: Physicochemical Properties: Trypsin products undergo testing to determine pH and osmolality according to specific protocols in which all equipment is calibrated daily using standards that are traceable to the National Institute of Standards and Technology. Biological Tests: Trypsin Solutions are formulated under controlled manufacturing conditions to avoid loss of enzymatic activity by denaturation. Sterility: Mediatech selects a representative number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP current edition. Mycoplasma: Trypsin is tested for mycoplasma off-site using a large-volume method described by Kern and Barile in "Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques" M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11.5. Razin and J.G. Tully, eds., New York: Academic Press, 159-165. Porcine Parvovirus: Powder must test negative by the Code of Federal Regulations, Title 9; 113.53-d. Activity: Finished product is tested according to predetermined specifications for its ability to remove adherent cultures without damaging cellular integrity. In vitro assays are performed on HEP-2/MRC-5 and VERO cultures to verify compliance with product specifications.
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Trypsin 1X, 25-050-CI
0.25% Trypsin in HBSS without calcium and magnesium Porcine Parvovirus tested
$48.96
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Trypsin EDTA 1X, 25-051-CI
0.05% Trypsin/0.53 mM EDTA in HBSS without calcium and magnesium Porcine Parvovirus Tested
$49.30
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Trypsin EDTA 1X, 25-053-CI
0.25% Trypsin/2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium Porcine Parvovirus Tested
$48.96
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Trypsin 10X, 25-054-CI
2.5% Trypsin in HBSS without calcium, magnesium, and phenol red Porcine Parvovirus Tested
$130.63
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Trypsin EDTA 1X, 25-052
0.05% Trypsin/0.53 mM EDTA in HBSS without sodium bicarbonate, calcium, and magnesium Porcine Parvovirus Tested
Starting at:
$49.02
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