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Enzymatic Cell Dissociation Agents

Trypsin:
Physicochemical Properties: Trypsin products undergo testing to determine pH and osmolality according to specific protocols in which all equipment is calibrated daily using standards that are traceable to the National Institute of Standards and Technology.

Biological Tests: Trypsin Solutions are formulated under controlled manufacturing conditions to avoid loss of enzymatic activity by denaturation.

Sterility: Mediatech selects a representative number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP current edition.

Mycoplasma: Trypsin is tested for mycoplasma off-site using a large-volume method described by Kern and Barile in "Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques" M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11.5. Razin and J.G. Tully, eds., New York: Academic Press, 159-165.

Porcine Parvovirus: Powder must test negative by the Code of Federal Regulations, Title 9; 113.53-d.

Activity: Finished product is tested according to predetermined specifications for its ability to remove adherent cultures without damaging cellular integrity. In vitro assays are performed on HEP-2/MRC-5 and VERO cultures to verify compliance with product specifications.

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Trypsin 1X
25-050-CI
$55.37
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0.25% Trypsin in HBSS without calcium and magnesium Porcine Parvovirus tested

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Trypsin EDTA 1X
25-051-CI
$54.43
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0.05% Trypsin/0.53 mM EDTA in HBSS with sodium bicarbonate, without calcium and magnesium, Porcine Parvovirus Tested.

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Trypsin EDTA 1X
25-053-CI
$54.04
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0.25% Trypsin/2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium Porcine Parvovirus Tested

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Trypsin 10X
25-054-CI
$144.21
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2.5% Trypsin in HBSS without calcium, magnesium, and phenol red Porcine Parvovirus Tested

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Accutase® Cell Detachment Solution, Liquid
25-058-CI
$38.22
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Technical Advantage:

Accutase® cell detachment solution contains proprietary marine-origin enzymes with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues. It’s useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including our selection of Advanced Surface coated plasticware. Accutase does not contain mammalian or bacterial derived products.


- Efficient, with gentle detachment of adherent cells within minutes

- An alternative to Trypsin

- Able to eliminate neutralization or wash steps

- Convenient and cost effective

- Complete Certificate of Analysis available for each production lot

- Produced under the highest industry standards to ensure superior results


Accutase is a registered trademark of Innovative Cell Technologies, Inc.

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Trypsin EDTA 1X
25-052
Starting at: $54.12

0.05% Trypsin/0.53 mM EDTA in HBSS without sodium bicarbonate, calcium, and magnesium Porcine Parvovirus Tested

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