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Insect Media and Reagents
Insect cell culture has become an increasingly important tool in biotechnology for the production of biologicals. The inherent growth characteristics of the insect cells make them ideal candidates for Baculovirus Expression Vector Systems (BEVS) for the production of recombinant genes because they provide advantages over both bacterial and mammalian cultures, due to their fast doubling times and ability to grow in a suspension. Insect cells have significantly impacted protein production through the use of large-scale bioreactors to produce recombiant proteins and products of medical and agricultural importance with high expression levels via BEVS. Conventional insect culture media rely heavily on animal sera and other components such as yeast hydrolysate, lactalbumin hydrolysate, tryptose phosphate broth, and hemolymph as the nutritional, chemical, and physiological support systems. However, the production of purified recombinant proteins has created a demand for media capable of supporting growth without relying on the additives.
